They found that hPL promoted faster BMSC proliferation and higher calcium content and alkaline phosphatase activity compared to FCS, especially in the early stages of differentiation. These results suggest hPL can replace FCS in culture media without affecting osteogenic differentiation.
According to the research, cells cultured in 10% hPL-supplemented expansion medium exhibited significantly higher (p < 0.01) population doublings, averaging 4.46 cumulative doublings, compared to cells in 10% FCS-supplemented expansion medium, which averaged 2.22 cumulative doublings. Researchers compared the performance regarding the proliferation rate and osteogenic differentiation of BMSC cultured in an expansion medium and osteogenic differentiation medium containing different concentrations of hPL or FCS, and evaluated the impact of the protein supplement’s concentration on the osteogenic differentiation of BMSC.
The study shows that hPL supplementation in the expansion medium doubles BMSC proliferation compared to FCS, and enhances osteogenic differentiation in a concentration-dependent manner. The effect is strongest at 10% hPL early in the differentiation period, accelerating osteogenic potential without compromising it.
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