hPL supplementation leads to more consistent osteoclastic differentiation of MCs compared to 10% FBS. In co-cultures, hPL allows resorption, especially at low concentrations. High concentrations of hPL promote osteogenic differentiation and bone-like matrix formation in MSC cultures and co-cultures. The research highlights that hPL not only accelerates osteogenic differentiation of mesenchymal stromal cells (MSCs) but also supports osteoclastic differentiation, thereby improving bone resorption and remodeling processes.
Researchers studied three concentrations of hPL as a xenogeneic-free and physiologically relevant alternative to FBS for osteoclastic differentiation in MC mono-cultures. They also examined hPL’s effect on osteoclastic differentiation in MC-MSC co-cultures and osteogenically stimulated MC-MSC co-cultures for in vitro bone remodeling models.
The study emphasizes the potential of hPL to serve as a xenogeneic-free supplement, aligning with ethical considerations and enhancing the reproducibility of in vitro bone models. By incorporating hPL, researchers can develop more reliable and ethically sound models for studying bone resorption and remodeling, which are crucial for understanding bone diseases and developing therapeutic strategies.
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